PerlPrimer is a free, open-source GUI application written in Perl that designs primers for standard PCR, bisulphite PCR, real-time PCR (QPCR) and sequencing. It aims to automate and simplify the process of primer design.
PerlPrimer's current features include the following:
- Calculation of possible primer-dimers
- Retrieval of genomic or cdna sequences from Ensembl (including both sequences automatically for QPCR)
- Ability to BLAST search primers using the NCBI server or a local server
- Results can be saved or optionally exported in a tab-delimited format that is compatible with most spreadsheet applications.
- ORF and CpG island detection algorithms
- Ability to add cloning sequences to primers, automatically adjusted to be in-frame
- QPCR primer design without manual intron-exon boundary entry
PerlPrimer calculates primer melting temperature using J. SantaLucia's extensive nearest-neighbour thermodynamic parameters. To adjust for the salt conditions of the PCR, PerlPrimer uses the empirical formula derived by von Ahsen, et al. (2001) and allows the user to specify the concentration of Mg2+, dNTPs and primers, or use standard PCR conditions. The result is a highly accurate prediction of primer melting temperature, giving rise to a maximum yeild of product when amplified.
PerlPrimer is written in Perl and Perl/Tk. In addition, for QPCR functionality PerlPrimer uses the open-source Spidey executable from NCBI, and restriction enzyme data from the REBASE project is used when adding cloning sites. The program is designed to be cross-platform compatible and has been developed and tested on both Microsoft Windows and GNU/Linux-based operating systems. Users have also reported success using the program under Mac OS X.
Please cite the reference below if this program is useful.
Marshall OJ. PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR. Bioinformatics 2004 20(15):2471-2472 [Pubmed]
Maelezo ya mchapishaji (Kiingereza)
PerlPrimer ni nini?
PerlPrimer ni programu ya chanzo huria ya Windows kutoka Owen Marshall.
Usakinishaji na mahitaji
Kuna visakinishi rasmi kwa 32-bit katika muundo wa EXE (NSIS). Kisakinishi kikuu kina ukubwa wa 3.4 MB. Usakinishaji unaomba haki za msimamizi. Kisakinishi kinaruhusu usakinishaji bila maswali (kimya).
Umaarufu na aina za faili
Kwenye Windows inasajili aina 1 ya faili, zikiwemo .ppr.
Faida / Hasara
Faida
- Chanzo huria: mtu yeyote anaweza kukagua na kujenga msimbo
- Usakinishaji kimya umeelezwa
- Faili ndogo ya kupakua (3.4 MB)
Hasara
- Hakuna toleo asili la ARM64
- 32-bit pekee
- Usakinishaji unahitaji haki za msimamizi
- Hakuna sasisho kwa zaidi ya mwaka mmoja
Kwa ufupi: PerlPrimer ni zana ya chanzo huria ya Windows kwa kompyuta za 32-bit. Masasisho yamepungua. Wasimamizi wa mifumo wanaweza kuisambaza bila maswali.
Je, ni salama kupakua PerlPrimer?
- Faili inatolewa na sourceforge.net, kikoa cha mchapishaji mwenyewe
- Kiungo cha kupakua kinatumia HTTPS
- Hashi ya SHA256 imechapishwa, hivyo unaweza kuthibitisha faili:
F911658B761F1CA1F09F9A6C11E4540120F7FD7E1A11B328FACD35D03E03E8BB· Fungua ripoti ya VirusTotal ya hashi hii - Kiungo kilijibu tulipokikagua mara ya mwisho (22 Oktoba 2026)
- Msimbo chanzo uko wazi kwa umma
- ExeAtlas haihifadhi, haifungi wala haibadilishi visakinishi
Mwongozo unaohusiana: Jinsi ya kukagua hashi ya SHA256 ya faili kwenye Windows
Matoleo ya karibuni
Toleo 1 ›| Toleo | Tarehe | Ukubwa | Faili |
|---|---|---|---|
| 1.1.21Jipya zaidi | 21 Novemba 2011 | 3.4 MB | 32-bit EXE (NSIS) |
Kusakinisha kwa mstari wa amri
Usakinishaji kimya ›winget install --id OwenMarshall.PerlPrimer -edownload /SMwongozo unaohusiana: Jinsi ya kutumia winget: sakinisha na sasisha programu
Taarifa
- Mchapishaji
- Owen Marshall
- Leseni
- GPL-2.0-or-later
- Bei
- Bure
- Inahitaji
- Windows 11 / 10
- Usanifu
- 32-bit
- Kisakinishi
- EXE (NSIS)
- Toleo
- 1.1.21
- Ilitolewa
- 21 Novemba 2011
- Iliongezwa kwenye orodha
- 2 Agosti 2025
- Matoleo yaliyoorodheshwa
- 1
- Tovuti
- perlprimer.sourceforge.net
- Kitambulisho cha winget
- OwenMarshall.PerlPrimer
Aina za faili inazofungua (1)
Ukadiriaji na maoni
Bado hakuna maoni. Kuwa wa kwanza kuikadiria.
Maswali yanayoulizwa mara kwa mara
Je, PerlPrimer ni bure?
Ndiyo. PerlPrimer ni programu ya chanzo huria chini ya leseni ya GPL-2.0-or-later.
Je, ni salama kupakua PerlPrimer?
Faili inapakuliwa kutoka sourceforge.net, ambayo ni ya mchapishaji mwenyewe; ExeAtlas haihifadhi wala haibadilishi faili. Linganisha hashi yake ya SHA256 na ile iliyoonyeshwa hapa, au fungua ripoti ya VirusTotal ya hashi hiyo.
Jinsi ya kusakinisha PerlPrimer kwa winget?
Fungua PowerShell au Windows Terminal na uendeshe amri ya winget iliyoonyeshwa kwenye ukurasa huu. winget hupakua kisakinishi rasmi na kukagua hashi yake ya SHA256 kabla ya kukiendesha.
Je, PerlPrimer inaweza kusakinishwa kimya?
Ndiyo. Ukurasa wa usakinishaji kimya unaonyesha amri kamili kwa kila kisakinishi.
Je, PerlPrimer inafanya kazi kwenye ARM (Windows on Arm)?
Hakuna toleo la ARM64; Windows 11 on Arm inaweza kuendesha toleo la 32-bit kupitia uigaji (emulation).