FlowJo is a software package for analyzing flow cytometry data. Files produced by modern flow cytometers are written in the Flow Cytometry Standard format with an .fcs file extension. FlowJo will import and analyze cytometry data regardless of which flow cytometer is used to collect the data.
In FlowJo, samples are organized in a "Workspace" window, which presents a hierarchical view of all the samples and their analyses (gates and statistics). Viewing an entire experiment in a Workspace permits organizing and managing complex cytometry experiments and produces detailed graphical reports. FlowJo's ability to automate repetitive operations facilitates the production of statistics tables and graphical reports when the experiment involves many samples, parameters and/or operations.
Within a workspace, samples can be grouped or sorted by various attributes such as the panel of antibodies with which they are stained, tissue type, or patient from whom they came. When an operation on a group is initiated, FlowJo can perform the same operation on every sample belonging to that group. Thus, you can apply a gate to a sample, copy it to the group, and that gate will be automatically placed on all samples in the group.
What is FlowJo?
FlowJo by FlowJo LLC is proprietary Windows software. The first release dates back to 1996.
Installation and requirements
The publisher provides EXE (Inno Setup) installers for 64-bit Windows. The main installer is 364.7 MB. Installation asks for administrator rights. A silent install is possible with the installer’s documented switches.
Updates and version history
The current version is 11.2.0.0, released on May 29, 2026. The version history goes back to March 28, 2025: 7 releases, all linked to the official files. That is about 6 releases a year.
Popularity and file types
Wikipedia has an article about it in 3 of our languages. On Windows it registers 1 file type, including .flowjo.
Pros / Cons
Pros
- Documented silent install
- Mature project (since 1996)
Cons
- Proprietary licence: check the terms and price
- No native ARM64 build
- Installation needs administrator rights
- Large download (364.7 MB)
In short: FlowJo is a Windows tool for 64-bit PCs. It is actively maintained. Administrators can deploy it unattended.
Is FlowJo safe to download?
- The file is served by downloads.bdaccessportal.com; check that it is the publisher’s official mirror
- The download link uses HTTPS
- A SHA256 hash is published, so you can verify the file:
4EAC8EE61481ED267BD63DE7DAF9074E90A70F680F8C6E0C52AAD9F772EBA646· Open the VirusTotal report for this hash - The link answered when we last checked it (October 22, 2026)
- ExeAtlas does not host, wrap or modify installers
Related guide: How to check a file’s SHA256 hash on Windows
Recent versions
All 7 versions ›7 versions over 1 year · about 6 a year
| Version | Date | Size | Files |
|---|---|---|---|
| 11.2.0.0Latest | May 29, 2026 | 364.7 MB | 64-bit EXE (Inno Setup) |
| 11.1.1.0 | February 23, 2026 | — | 64-bit EXE (Inno Setup) |
| 11.1.0.0 | November 10, 2025 | — | 64-bit EXE (Inno Setup) |
| 11.0.2.0 | August 12, 2025 | — | 64-bit EXE (Inno Setup) |
| 11.0.1.0 | July 11, 2025 | — | 64-bit EXE (Inno Setup) |
| 11.0.0.0 | May 27, 2025 | — | 64-bit EXE |
| 10.10.0.0 | March 28, 2025 | — | 64-bit EXE |
Install from the command line
Silent install ›winget install --id FlowJo.FlowJo -eFlowJo-11.2.0-installer.exe /VERYSILENT /SUPPRESSMSGBOXES /NORESTART /SP-Related guide: How to use winget: install and update apps
Information
- Publisher
- FlowJo LLC
- Licence
- Proprietary
- Price
- See the publisher’s site
- Requires
- Windows 11 / 10
- Architecture
- 64-bit
- Installer
- EXE (Inno Setup)
- Version
- 11.2.0.0
- Released
- May 29, 2026
- First release
- 1996
- Added to the catalogue
- March 28, 2025
- Versions listed
- 7
- Updates / year
- 6
- Website
- flowjo.com
- winget ID
- FlowJo.FlowJo
File types it opens (1)
Ratings and reviews
No reviews yet. Be the first to rate it.
Frequently asked questions
Is FlowJo free?
FlowJo is proprietary (closed-source) software (licence: Proprietary). Whether it costs money depends on the edition: check the publisher’s site for prices and free tiers.
Is FlowJo safe to download?
The download comes from downloads.bdaccessportal.com; ExeAtlas does not host or change the file. Compare its SHA256 hash with the one shown here, or open the VirusTotal report for that hash.
How do I install FlowJo with winget?
Open PowerShell or Windows Terminal and run the winget command shown on this page. winget downloads the official installer and checks its SHA256 hash before running it.
Can FlowJo be installed silently?
Yes. The silent install page lists the exact command for each installer.
Does FlowJo run on ARM (Windows on Arm)?
There is no ARM64 build; Windows 11 on Arm can run the 64-bit version through emulation.
Where can I download an older version of FlowJo?
The versions page lists all 7 versions with links to their official installer files, back to March 28, 2025.